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edta [2026/01/08 02:40] – Scott Larsonedta [2026/02/08 19:24] (current) – Scott Larson
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 chelation agent for removing corneal calcium deposits chelation agent for removing corneal calcium deposits
  
-====Preparation methods====+====Preparation method 1 ====
   - 0.3 ml sterile water injected into a lavender top, 3.0 ml K2-EDTA BD Vacutainer CBC tube (each tube has 5.4 mg K2-EDTA)   - 0.3 ml sterile water injected into a lavender top, 3.0 ml K2-EDTA BD Vacutainer CBC tube (each tube has 5.4 mg K2-EDTA)
-  - Swirl the liquid around to dissolve the droplets of liquid in the container  +  - Swirl the liquid around to dissolve the coating in the container  
-  - Aspirate the contents and add it to a second lavender top tube, repeat step 2 +  - Poor the contents into a second lavender top tube (will have to remove the tops of both tubes), repeat step 2 
-  - Repeat for a total of 5 tubes +  - Repeat step 3 for a total of 5 tubes  
-  - results in 27 mg of K2-EDTA concentration of 9%, pH 5.8, +  - results in 65 mg/ml of K2-EDTA, pH 5.8, 
   - [[http://links.lww.com/ICO/A633|Video example]]   - [[http://links.lww.com/ICO/A633|Video example]]
 +====Peparation method 2 ==== 
 +  - 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA) 
 +  - Swirl the liquid around to dissolve the coating of the container  
 +  - results in about 35 mg/ml K2-EDTA  
 +====Preparation method 3 ==== 
 +  - 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA) 
 +  - Use a sterile cotton tip applicator to rub off all of the EDTA into the liquid 
 +  - Use the cotton tip applicator to transfer the EDTA to the cornea 
 +  - results in about 52 mg/ml K2-EDTA 
 +  - [[https://cdn-links.lww.com/permalink/ico/b/ico_2020_11_17_narvaez_1_sdc1.mp4|Video Example]]
 ====References==== ====References====
   - [[https://1drv.ms/b/c/31d83ae8e55e0542/IQDv2isZOfgDQZX0Tp-gCQCJAWr5065taet4Q_9zVMKZTes?e=q60Y6X|"Off-the-Shelf" K2-EDTA for Calcific Band Keratopahy. Lee et al. Cornea 2018;37(7):916-918]]   - [[https://1drv.ms/b/c/31d83ae8e55e0542/IQDv2isZOfgDQZX0Tp-gCQCJAWr5065taet4Q_9zVMKZTes?e=q60Y6X|"Off-the-Shelf" K2-EDTA for Calcific Band Keratopahy. Lee et al. Cornea 2018;37(7):916-918]]
   - [[https://1drv.ms/b/c/31d83ae8e55e0542/IQDLPKIpqwgARYqjxoi62JxkAQknFGQfWwO9n9HK23PO7T4?e=KnQpbO|Simplified, Readily Available Method for the Treatment of Band Keratopathy With Ethylenediaminetetraacetic Acid. Narvaez J. et al. Cornea 2021;40(10):1360-1362]]    - [[https://1drv.ms/b/c/31d83ae8e55e0542/IQDLPKIpqwgARYqjxoi62JxkAQknFGQfWwO9n9HK23PO7T4?e=KnQpbO|Simplified, Readily Available Method for the Treatment of Band Keratopathy With Ethylenediaminetetraacetic Acid. Narvaez J. et al. Cornea 2021;40(10):1360-1362]]