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| retinoblastoma_fresh_tumor_specimen [2015/06/29 03:28] – created admin | retinoblastoma_fresh_tumor_specimen [2025/05/22 22:51] (current) – Scott Larson | ||
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| + | ====== Retinoblastoma-Fresh Tumor Specimen ====== | ||
| + | * Use 25 gauge 5/8 inch needle on 5cc syringe. | ||
| + | * Pull back with some force, don't put air in the eye | ||
| + | * Look for some tissue, if vitreous comes, eye may flatten | ||
| + | * Can fill the eye with some saline and 30g if it flattens | ||
| + | * Remove tenons over injection site to prevent trapping cells | ||
| + | * Wash eye with saline after pulling out tissue | ||
| + | * Ensure the tumor cells are in transport media- small white floaters | ||
| + | * Place on Path specimen report that eye was punctured for cell culture | ||
| + | * See details on [[retinoblastoma_genetic_testing|UIHC Epic Ordering]] | ||
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| + | {{tag> | ||