Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision | |||
| edta [2026/02/08 19:22] – Scott Larson | edta [2026/02/08 19:24] (current) – Scott Larson | ||
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| Line 9: | Line 9: | ||
| - Poor the contents into a second lavender top tube (will have to remove the tops of both tubes), repeat step 2 | - Poor the contents into a second lavender top tube (will have to remove the tops of both tubes), repeat step 2 | ||
| - Repeat step 3 for a total of 5 tubes | - Repeat step 3 for a total of 5 tubes | ||
| - | - results in 27 mg of K2-EDTA | + | - results in 65 mg/ml of K2-EDTA, pH 5.8, |
| - [[http:// | - [[http:// | ||
| ====Peparation method 2 ==== | ====Peparation method 2 ==== | ||
| - 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA) | - 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA) | ||
| - Swirl the liquid around to dissolve the coating of the container | - Swirl the liquid around to dissolve the coating of the container | ||
| + | - results in about 35 mg/ml K2-EDTA | ||
| ====Preparation method 3 ==== | ====Preparation method 3 ==== | ||
| - 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA) | - 0.3 ml sterile water injected into a 10 ml lavender top K2-EDTA BD Vacutaner tube (each tube has 18 mg K2-EDTA) | ||
| - Use a sterile cotton tip applicator to rub off all of the EDTA into the liquid | - Use a sterile cotton tip applicator to rub off all of the EDTA into the liquid | ||
| - Use the cotton tip applicator to transfer the EDTA to the cornea | - Use the cotton tip applicator to transfer the EDTA to the cornea | ||
| + | - results in about 52 mg/ml K2-EDTA | ||
| - [[https:// | - [[https:// | ||
| ====References==== | ====References==== | ||